Journal: MethodsX

Article Title: Biofluids manipulation methods for liquid biopsy in minimally-invasive assays

doi: 10.1016/j.mex.2022.101759

Figure Lengend Snippet: Biomarkers from saliva, urine, stool, seminal fluid and handling methods.

Article Snippet: If the sample is destined to DNA extraction has to be shipped, storage on dry ice is required otherwise, a commercial brand stabilizer (DNA Genotek P-084 for DNA extraction, DNA Genotek P-085 for DNA and RNA extraction, Stratec PSP Spin stool DNA plus kit, brown Sarstedt) may be added to the sample after collection, mixed vigorously and shipped at room temperature.

Techniques: Biomarker Discovery, Centrifugation, Sterility, Bacteria, Extraction, Lysis

Journal: Redox Biology

Article Title: Determination of protein carbonyls in plasma, cell extracts, tissue homogenates, isolated proteins: Focus on sample preparation and derivatization conditions

doi: 10.1016/j.redox.2015.06.005

Figure Lengend Snippet: Measurements of protein carbonyls by immunoblotting.

Article Snippet: , 5 µg/ml , 45 min at RT in the dark , Anti-rabbit HRP-linked IgG (H&L, Upstate Cell Signaling) , , , .

Techniques: Western Blot, Sample Prep, Plasmid Preparation, Purification, Lysis, Dot Blot, Semi Dry Blot, Isolation, Knock-Out, Activity Assay

Journal: Redox Biology

Article Title: Determination of protein carbonyls in plasma, cell extracts, tissue homogenates, isolated proteins: Focus on sample preparation and derivatization conditions

doi: 10.1016/j.redox.2015.06.005

Figure Lengend Snippet: Determination of protein carbonyls by ELISA.

Article Snippet: , 5 µg/ml , 45 min at RT in the dark , Anti-rabbit HRP-linked IgG (H&L, Upstate Cell Signaling) , , , .

Techniques: Enzyme-linked Immunosorbent Assay, Sample Prep, Concentration Assay, Modification, Lysis, Derivative Assay, Isolation, Infection

Journal: Pharmaceutics

Article Title: Novel Approaches for the Treatment of Pulmonary Tuberculosis

doi: 10.3390/pharmaceutics12121196

Figure Lengend Snippet: Pulmonary drug delivery strategies in the treatment of tuberculosis.

Article Snippet: To investigate inhalable dry powder AERAS-402 vaccine in term of physical stability and aerodynamic properties , Pulmonary , Dry powder inhaler , Microcarriers , Spray drying method , Mannitol sucrose β -Cyclodextrin Dextran Leucine PVP , NA , MCTD formulation showed the most thermostability stabilizer with Tg value of 97.09 °C, which was the highest among all prepared formulations. Desired aerodynamic characteristics were demonstrated in mannitol-based formulations with narrow size distribution (3.2–3.5 μm). MCTD formulation had higher resistance to water adsorption. MCTD dry powder AERAS-402 showed good stability with 0.12 log loss of virus activity after 5 weeks of storage at 37 °C. , [ ] .

Techniques: Formulation, Solvent, Concentration Assay, Clinical Proteomics, Aerosol, Activity Assay, Infection, Maltodextrin, Molecular Weight, Homogenization, In Vivo, Solubility, Crystallization Assay, In Vitro, Zeta Potential Analyzer, Suspension, Emulsion, Evaporation, Produced, Lyophilization, Liposomes, Adjuvant, Animal Model, Adsorption, Virus